A hybridization probe is a fragment of DNA or RNA of variable length which is usually used in DNA or RNA samples to detect the presence of nucleo...
A hybridization probe is a fragment of DNA or RNA of variable length which is usually used in DNA or RNA samples to detect the presence of nucleotide sequences that are complementary to the sequence in the probe. Because of the complementarity between the probe and nucleotide sequences, the probe can hybridize to single-stranded nucleic acid. The labeled probe is first denatured by some lab techniques into single stranded DNA and then hybridized to the target single stranded DNA or RNA which is immobilized on a membrane or in situ.
The probe is labeled with a molecular marker of either radioactive or fluorescent molecules, thus helping easily detect the hybridization of the probe to its target sequence. DNA sequences or RNA transcripts with moderate or high sequence similarity to the probe will be detected by visualizing the hybridized probe via autoradiography or other imaging techniques.
DNA probes are used to detect a specific gene in a long DNA sequence and can provide diagnosis of infections and disease, identify food contaminants and determine the presence of microbial species. They are the powerful new tools for medical and veterinary diagnostic laboratories, helping to enhance the diagnosis of infectious diseases, genetic disorders, and malignancies.
RNA probes can be used for applications, such as Northern blotting, RNase protection assays, Southern blotting, downstream of polymerase chain reaction (PCR), and in situ hybridization analysis. They are usually produced by transcription from cloned DNA, and now are preferred for genetic studies compared to the applications of DNA probes. They can be further divided into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.
The US based genomic products supplier, Creative Bioarray, provides a comprehensive list of FISH probes for rapid identification of a wide range of chromosomal aberrations across the genome, including Chromosome Probes, Diagnostic Probes, Animal Probes, and Bacteria Probes. Those probes target relevant regions for a wide range of applications such as detection of gene amplification, deletion, translocation and chromosomal aneuploidies associated with tumor and genetic disease profiling.
Technical Strategies for Targeted Sequencing and Its Present and Future
The exon group, for example, accounts for only 1% to 2% of the genome but 85% of the known mutations. So, compared with whole genome sequencing (WGS), exon sequencing is equivalent to a 98% reduction in sequencing. Needless to say, targeting sequencing collection (panel) is a great way to lighten your burdens. Here we look at how researchers conduct targeted enrichment and the factors they consider in their research.
Difference Between Western Blot and ELISA for HIV Test
There are many techniques of knowing the presence of the virus in human body and out of them ELISA and Western Blot are very popular today.
Oligonucleotide Synthesis in Molecular Biology
In a strict sense, an oligonucleotide is a short piece of DNA or RNA which is less than 50 nucleotides in length. But in a broad sense, it also refers to all chemically synthesized nucleic acids, regardless of the length.
